Supplementary Figures and Tables for "Diversity, activity and abundance of benthic microbes in the Southeastern Mediterranean Sea", Rubin-Blum et al.

Name: Supplementary Figures and Tables for "Diversity, activity and abundance of benthic microbes in the Southeastern Mediterranean Sea", Rubin-Blum et al.
Creator: Rubin Blum, Maxim
Published: 2022-01-13 00:00:00
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Figshare2022-01-13 更新2026-04-08 收录

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Figures: Figure 1: Alpha diversity parameters. Abundance-based coverage estimator – ACE (bacteria and archaea, A; fungi, C) and Simpson's diversity index (bacteria and archaea, B; fungi, D). cmbsf – cm below the seafloor. Figure 2: Rarefaction curves – bacteria and archaea. Samples collected in 2019, which were sequenced to a considerably lower depth, were excluded from alpha-diversity analyses. Figure 3: Rarefaction curves – bacteria and archaea. Samples collected in 2019, which were sequenced to a considerably lower depth, were excluded from alpha-diversity analyses. Figure 4: Determining optimal k-means clusters dor sediment samples from the 0-1 cm sections, using 3 methods: gap statistic (top), average silhouette (middle) and elbow (bottom). 5 to 8 optimal clusters are suggested. Figure 5: Read abundance of top, 30 most-abundant bacterial and archaeal lineages at the order level in deep plain sediments. Taxa are hierarchically clustered. Figure 6: Read abundance of top, 30 most-abundant bacterial and archaeal lineages at the order level in continental slope sediments. Taxa are hierarchically clustered. Figure 7: Read abundance of top, 30 most-abundant bacterial and archaeal lineages at the order level in continental shelf sediments. Taxa are hierarchically clustered. Figure 8: Read abundance of top, 30 most-abundant bacterial and archaeal lineages at the order level coastal sediments. Taxa are hierarchically clustered. Figure 9: Read abundance of the 30 most abundant fungi orders in coastal sediments (UNITE BLAST). Figure 10: Read abundance of the 30 most abundant fungi orders in continental shelf sediments (UNITE BLAST). Figure 11: Read abundance of the 30 most abundant fungi orders in continental shelf sediments (UNITE BLAST). Figure 12: Read abundance of the 30 most abundant fungi orders in the deep sediments. (UNITE BLAST). Figure 13: Spearman's rank correlation coefficient (Spearman's ρ) for the bacterial and archaeal taxa read abundancies (order level, top 30) and environmental parameters. *,**, *** correspond to the adjusted p values (<0.05, <0.005, <0.0005). Figure 14: Spearman's rank correlation coefficient (Spearman's ρ) for the fungal taxa read abundancies (order level, top 30) and environmental parameters. *,**, *** correspond to the adjusted p values (<0.05, <0.005, <0.0005). Supplementary Tables: Table S1: DSEq2 analyses showing bacterial and archaeal taxa (order level), enriched in deep, slope, shelf and coastal habitats. Table S2: Indicator species analyses showing bacterial and archaeal taxa (order level), enriched in deep, slope, shelf and coastal habitats. Table S3: DSEq2 analyses showing bacterial and archaeal taxa (order level) that correlate with changes in distance from the sediment surface. Table S4: DSEq2 analyses showing fungal taxa (order level), enriched in deep, slope, shelf and coastal habitats. Table S5: Indicator species analyses showing fungal taxa (order level), enriched in deep, slope, shelf and coastal habitats. Table S6: DSEq2 analyses showing fungal taxa (order level) that correlate with changes in distance from the sediment surface.

图表： 图1：α多样性（alpha diversity）参数。基于丰度的覆盖度估计量（Abundance-based coverage estimator，ACE）——细菌与古菌对应A、真菌对应C；辛普森多样性指数（Simpson's diversity index）——细菌与古菌对应B、真菌对应D。cmbsf——海底以下厘米数（cm below the seafloor）。 图2：稀疏曲线（rarefaction curves）——细菌与古菌。2019年采集、测序深度显著较低的样本被排除在α多样性分析之外。 图3：稀疏曲线（rarefaction curves）——细菌与古菌。2019年采集、测序深度显著较低的样本被排除在α多样性分析之外。 图4：针对0-1cm层位的沉积物样本，采用3种方法确定最优k均值聚类（k-means）簇：间隙统计量（gap statistic，上图）、平均轮廓系数（average silhouette，中图）与肘部法则（elbow，下图），建议最优聚类数为5至8。 图5：深海平原沉积物中，丰度排名前30的细菌与古菌分类群（目级水平）的读长丰度。分类群已进行层级聚类。 图6：大陆坡沉积物中，丰度排名前30的细菌与古菌分类群（目级水平）的读长丰度。分类群已进行层级聚类。 图7：大陆架沉积物中，丰度排名前30的细菌与古菌分类群（目级水平）的读长丰度。分类群已进行层级聚类。 图8：海岸沉积物中，丰度排名前30的细菌与古菌分类群（目级水平）的读长丰度。分类群已进行层级聚类。 图9：海岸沉积物中，丰度排名前30的真菌目级分类群的读长丰度（UNITE BLAST）。 图10：大陆架沉积物中，丰度排名前30的真菌目级分类群的读长丰度（UNITE BLAST）。 图11：大陆架沉积物中，丰度排名前30的真菌目级分类群的读长丰度（UNITE BLAST）。 图12：深海沉积物中，丰度排名前30的真菌目级分类群的读长丰度（UNITE BLAST）。 图13：细菌与古菌分类群（目级水平，前30位）的读长丰度与环境参数间的斯皮尔曼等级相关系数（Spearman's rank correlation coefficient，Spearman's ρ）。*、**、***分别对应校正后p值（<0.05、<0.005、<0.0005）。 图14：真菌分类群（目级水平，前30位）的读长丰度与环境参数间的斯皮尔曼等级相关系数（Spearman's rank correlation coefficient，Spearman's ρ）。*、**、***分别对应校正后p值（<0.05、<0.005、<0.0005）。 补充表格： 表S1：DSEq2分析结果，显示在深海、大陆坡、大陆架与海岸生境中富集的细菌与古菌分类群（目级水平）。 表S2：指示物种分析结果，显示在深海、大陆坡、大陆架与海岸生境中富集的细菌与古菌分类群（目级水平）。 表S3：DSEq2分析结果，显示与沉积物表层距离变化相关的细菌与古菌分类群（目级水平）。 表S4：DSEq2分析结果，显示在深海、大陆坡、大陆架与海岸生境中富集的真菌分类群（目级水平）。 表S5：指示物种分析结果，显示在深海、大陆坡、大陆架与海岸生境中富集的真菌分类群（目级水平）。 表S6：DSEq2分析结果，显示与沉积物表层距离变化相关的真菌分类群（目级水平）。

提供机构：

Rubin Blum, Maxim

创建时间：

2022-01-13