RNA-seq of H1 (WA-01) hESCs cultured in 3 conditions (mTeSR1, 4iLA + feeders and FINE)

RNA-seq was performed to look at global differences in gene expression between human embryonic stem cells (hESCs) particularly H1 cell line (WA-01, passage 23-40) cultured in distinct conditions. In details we compared cells adapted to primed (mTeSR1) and two naïve states: feeder dependent (4iLA + feeder) and feeder free (FINE) states.

本研究通过RNA测序（RNA-seq）探究人类胚胎干细胞（human embryonic stem cells, hESCs）在不同培养条件下的全基因组基因表达差异，实验所用细胞为特定的H1细胞系（WA-01，传代23-40代）。具体而言，本研究比较了适应始发态（primed）培养体系（mTeSR1培养基）的细胞，以及两种初始态（naïve）干细胞：饲养层依赖型（4iLA + 饲养层细胞）和无饲养层型（FINE培养体系）的细胞。