Detection limit of the MIP assay.

The MIP (MIPFOC01) was mixed with different amounts of Foc genomic DNA (160 ng, 80 ng, 40 ng, 20 ng, 10 ng, 5 ng, 2.5 ng and 1.25 ng) in triplicates and analyzed in a MIP assay. The PCR amplicon was electrophoresed through an agarose gel and the intensity of the DNA bands were measured. Error bars represent the standard deviation of three replicates.

将MIP（MIPFOC01）与不同梯度剂量的Foc基因组DNA（160 ng、80 ng、40 ng、20 ng、10 ng、5 ng、2.5 ng及1.25 ng）以三次重复方式混合，随后开展MIP检测实验并完成分析。将所得PCR扩增子通过琼脂糖凝胶电泳分离，并对DNA条带的信号强度进行定量测定。误差棒代表三次重复的标准差。