RNA Sequencing of C elegans control and mutant worms after 24 hour P.aeruginosa infection

Purpose: After infection there was significant decrease in the lifespan of mutant animals . To decipher the mechanism responsible and the changes in the gene expression of mutant worms Methods: Control and mutant worms were exposed at young adult stage to P.aeruginosa PA14 infection for 24 hours. Total RNA was extracted and RNA sequencing was done following standard protocols Results: Detailed analysis of the transcriptomic data show that immune genes were significantly upregulated which have role in lifespan Conclusions: This study showed the role of pathogenic infection in the life span modulation Overall design: C. elegans RNA seq of young adults of control and mutant worms after 24 hour P.aeruginosa PA14 infection in triplicate, using Illumina platform

研究目的：感染后突变型动物的寿命显著缩短。本研究旨在解析其背后的作用机制，以及突变型线虫的基因表达变化情况。
实验方法：将年轻成虫阶段的对照组与突变型线虫暴露于铜绿假单胞菌（Pseudomonas aeruginosa）PA14菌株的感染环境中，处理时长24小时。随后按照标准实验流程提取总RNA并进行RNA测序（RNA sequencing）。
实验结果：对转录组数据的详细分析显示，与寿命调控相关的免疫基因出现显著上调表达。
研究结论：本研究证实了病原感染在线虫寿命调控中发挥的重要作用。
实验整体设计：采用Illumina测序平台，对感染铜绿假单胞菌PA14菌株24小时后的对照组与突变型年轻成虫秀丽隐杆线虫（C. elegans）进行RNA测序，实验设置三次生物学重复。