The effect of Wolbachia on the distrubution of virus-derived 21nt interfering RNAs in Jw18 cells.

Distribution of 21 nt long viRNAs along sense (upper bars, black; 5’-3’ orientation) or antisense (lower bars, red; 3′-5′ orientation) strands of the SFV genome in the absence (A) (Jw18Free) or presence (B) (Jw18Wol) of Wolbachia. A zoomed in version of (B) is shown in (C) for ease of comparison. Data are from 24 hpi with SFV4(3H)-RLuc, of Jw18 D. melanogaster cells. Concatenated data from 5 independent infections are shown in all panels.

本数据集展示了21核苷酸长度的病毒小RNA（viRNA）在塞姆利基森林病毒（SFV）基因组正义链与反义链上的分布情况：正义链对应上方黑色条带，方向为5’-3’；反义链对应下方红色条带，方向为3’-5’。其中组A为未感染沃尔巴克氏体（Wolbachia）的Jw18Free样本，组B为感染沃尔巴克氏体的Jw18Wol样本。为便于对比，图C展示了组B的放大视图。所有数据均来自以SFV4(3H)-RLuc感染Jw18黑腹果蝇（D. melanogaster）细胞后24小时（感染后小时数，hpi）的样本，所有图组均采用5次独立感染实验的合并数据。