HPLC and fluorometric derived phytoplankton pigment concentrations from seawater collected on BATS Validation cruises from June 1996 to July 2024

Data presented here are discrete bottle HPLC derived phytoplankton pigments and fluorometric chlorophyll-a for BATS validation (BVAL) cruises from June 1996 (BVAL cruise #50016) through June/July 2024 (BVAL cruise #50061). Water samples are typically collected from 7-12 depths in the upper 250 meters of the water column, and then filtered under low vacuum through a 25mm GF/F filter. The filter is then flash frozen in liquid nitrogen and stored at -80 degrees C. Shoreside, analysis is performed on an HPLC using a method modified by Dr. R. Bidigare from the Wright et al. (1991) procedure. This method identifies the pigments chlorophyll-c3, chlorophyll-c2, peridinin, 19’-butanoyloxyfucoxanthin, fucoxanthin, 19’-hexanoyloxyfucoxanthin, prasinoxanthin, diadinoxanthin, alloxanthin, diatoxanthin, lutein, zeaxanthin, chlorophyll-b, chlorophyll-a, divinyl chlorophyllide-a, alpha and beta carotene. Additionally, chlorophyll-a and phaeopigments are analyzed using a fluorometric assay.

本数据集涵盖1996年6月（BVAL航次#50016）至2024年6/7月（BVAL航次#50061）期间，所有用于百慕大大西洋时间序列研究（BATS）验证的BVAL航次的离散瓶采样高效液相色谱（High Performance Liquid Chromatography, HPLC）法浮游植物色素数据，以及荧光法叶绿素a数据。水样通常采集自水柱上层250米的7~12个深度层，随后在低真空条件下通过25mm GF/F滤膜进行过滤。滤膜经液氮快速冷冻后，置于-80℃环境中保存。岸基实验室采用由R.比迪加尔（R. Bidigare）博士基于Wright等人1991年的实验方法改良后的HPLC分析法开展检测，该方法可鉴定的色素包括：叶绿素c3、叶绿素c2、多甲藻素（peridinin）、19'-丁酰氧基岩藻黄素（19’-butanoyloxyfucoxanthin）、岩藻黄素（fucoxanthin）、19'-己酰氧基岩藻黄素（19’-hexanoyloxyfucoxanthin）、绿藻黄素（prasinoxanthin）、硅藻黄质（diadinoxanthin）、别藻黄素（alloxanthin）、双硅藻黄质（diatoxanthin）、叶黄素（lutein）、玉米黄质（zeaxanthin）、叶绿素b（chlorophyll-b）、叶绿素a（chlorophyll-a）、二乙烯基叶绿素酸酯a（divinyl chlorophyllide-a）、α-胡萝卜素与β-胡萝卜素（alpha and beta carotene）。此外，本数据集还包含采用荧光分析法测得的叶绿素a及脱镁色素数据。