C-type lectin receptor expression is a hallmark of neutrophils infiltrating the skin in epidermolysis bulls acquisita.. C-type lectin receptor expression is a hallmark of neutrophils infiltrating the skin in epidermolysis bulls acquisita.

Characterization of neutrophil activation from blood to blister in experimental epidermolysis bullosa acquisita: C-type lectin receptors are dispensable in inducing disease. Overall design: Mice aged 6-8 weeks were used for the experiments (n=3 / cohort). Mice were treated with thrice weekly injections of 250ug of antigen affinity purified vWFA2-COL7 IgG or control rabbit IgG for 2 weeks. Tissue harvesting was subsequently performed after this 2 week period. Single cell suspensions of whole blood (WB) cells or ear skin cells were pooled per cohort.

实验性获得性大疱性表皮松解症中从血液到水疱的中性粒细胞活化特征：C型凝集素受体（C-type lectin receptors）在疾病诱导过程中并非必需。
实验设计：选用6-8周龄小鼠开展本实验，每个队列n=3。小鼠每周接受三次注射，注射剂量为250μg抗原亲和纯化的vWFA2-COL7 IgG或对照兔IgG，连续给药2周。于2周给药周期结束后采集组织样本。将每个队列的全血（whole blood, WB）细胞或耳皮肤细胞制备为单细胞悬液并进行合并。