A LncRNA-MAF/MAFB transcription factor network regulates epidermal differentiation [expression]

Using kinetic gene expression analysis of regenerated epidermis over a 7 day time-course, we identify a lncRNA-transription factor network with the MAF/MAFB transcription factors as central players in directing the epidermal differentiation program. Gene expression analysis: To establish a differentiation signature for regenerated epidermis over a 7 day time course and assess the effect of MAF/MAFB depletion in regenerated epidermis; total RNA was isolated in biologic duplicate from regenerated tissue in different conditions and hybridized to Affymetrix HGU133 2.0 arrays.

本研究通过对7天时间进程内再生表皮开展动态基因表达分析，鉴定出一个以MAF/MAFB转录因子为核心调控表皮分化程序的长链非编码RNA（long non-coding RNA，lncRNA）-转录因子调控网络。
基因表达分析：为构建7天时间进程下再生表皮的分化特征谱，并评估MAF/MAFB基因敲降对再生表皮的影响，我们从不同处理条件的再生组织中提取生物学重复的总RNA，将其与Affymetrix HGU133 2.0基因芯片进行杂交。