Expression data from murine splenic erythroblasts in stress erythropoiesis.. Mus musculus

In absence of selenium, proerythroblasts exhibited delay in differentation into basophilic erythroblasts during phenylhydrazine induced stress erythropoiesis. This microarray project was aiming to explore gene expression patterns in erythroblasts in the function of selenium status. Overall design: Proerythroblasts and basophilic erythroblasts were FACS sorted from spleen of selenium deficient or adequate mice on day 3 post phenylhydrazine treated mice. RNA was extracted and processed for microarray.

在硒缺乏条件下，苯肼（phenylhydrazine）诱导的应激性红细胞生成（stress erythropoiesis）过程中，原红细胞（proerythroblast）向嗜碱性成红细胞（basophilic erythroblast）的分化进程出现延迟。
本基因芯片（microarray）项目旨在探究不同硒状态下成红细胞的基因表达谱。
总体实验设计：对经苯肼处理3天后的硒缺乏与硒充足小鼠的脾脏，通过荧光激活细胞分选（FACS）分离得到原红细胞与嗜碱性成红细胞；
提取总RNA并进行基因芯片检测分析。