Transcriptomic analyis of SVG-p12 human fetal glial cells with overexpression of wildtype SOX10 or schwannoma-derived mutant isoforms

To investigate the gene expression changes caused by wildtype versus schwannoma-derived mutations in SOX10, we established SVG-p12 cell lines stably expressing either empty pCDF1 vector, wildtype SOX10, or two different mutant isoforms of SOX10. We then performed gene expression profiling analysis using RNA-seq for these SVG-p12 cell lines stably transduced with wildtype or mutant SOX10 isoforms. Overall design: Comparative gene expression profiling analysis of RNA-seq data for SVG-p12 cells following stable transduction with empty pCDF1 vector, wildtype SOX10, or two different tumor-derived mutant isoforms of SOX10.

为探究SOX10基因的野生型与雪旺细胞瘤来源的突变所诱导的基因表达变化，我们构建了稳定表达空pCDF1载体、野生型SOX10，以及两种不同突变型SOX10同工型的SVG-p12细胞系。随后，我们对经稳定转导野生型或突变型SOX10同工型的SVG-p12细胞系，采用RNA测序（RNA-seq）开展基因表达谱分析。整体实验设计：针对稳定转染空pCDF1载体、野生型SOX10，或两种不同肿瘤来源的SOX10突变同工型的SVG-p12细胞的RNA-seq数据，进行对比基因表达谱分析。