The miRNA profiles of plasma extracellular vesicles (pEV) in melanoma patients and supernatant EV (sEV) of related cell cultures

The dataset contains miRNA pEV profiles measured on Agilent Microarrays from three groups of melanoma patients (before or at resection, and after resection categorized into high-risk and low-risk groups) and healthy controls. These groups were compared to find differentially abundant miRNAs in the pEV of melanoma patients. In addition, two comparisons of short-term profile changes around the resection date are included, as well as six cell line sEV that play a role for the accompanying publication. The four groups (14 healthy controls and 14 tumor, 15 high-risk, 11 low-risk patients) were subjected to differential expression analysis. The additional pEV samples served illustrational purposes as outgroup comparisons, and the six cell line samples were employed in an attempt to elucidate the putative source tissue of the pEV. -------------------------------------- There are two subsets: the first one consists of the above mentioned patient samples and was used for the differential expression analysis in melanoma context, which is why there are only 54 columns in the normalized matrix. The remainder of the samples were used in very specific comparative analyses with the groups from the first subset. These two approaches have completely different scopes and subgroup-dependent normalizations were performed as necessary.

本数据集包含通过安捷伦微阵列（Agilent Microarrays）检测得到的微小RNA（microRNA, miRNA）血浆细胞外囊泡（plasma extracellular vesicle, pEV）表达谱，样本来源包括三组黑色素瘤患者与健康对照人群：三组黑色素瘤患者分别为术前/手术时患者、术后分为高危组与低危组的患者。本研究通过组间比较，旨在筛选黑色素瘤患者pEV中差异丰度显著的miRNA。
此外，数据集还包含两项针对切除术前后短期表达谱变化的比较分析样本，以及6株细胞系来源的小细胞外囊泡（small extracellular vesicle, sEV）样本，用于配套发表的学术研究。
其中四组核心样本（14例健康对照、14例肿瘤患者、15例高危患者、11例低危患者）被用于差异表达分析。其余额外的pEV样本作为外组对照以起到示例说明的作用，而6株细胞系sEV样本则用于阐明pEV的潜在来源组织。
本数据集包含两个子数据集：第一个子数据集由上述提及的患者样本组成，用于黑色素瘤相关的差异表达分析，因此其标准化表达矩阵仅包含54个样本列。剩余样本则与第一个子数据集的各组样本开展特定的比较分析。两种分析思路的研究范围完全不同，且根据研究需要分别进行了基于亚组的标准化处理。