Distinct Expression Profiles of long non-coding RNAs and messenger RNAs in circulating leukocytes of Parkinson’s disease. Distinct Expression Profiles of long non-coding RNAs and messenger RNAs in circulating leukocytes of Parkinson’s disease

The molecular mechanism underlying Parkinson's disease (PD), an increasingly common neurodegenerative disease, remains unclear. Long non-coding RNA (lncRNA) plays essential roles in gene expression and human disease. We hypothesize that lncRNAs are involved in the pathogenesis of PD. We profiled the expression of lncRNA in circulating leukocytes of 5 PD patients and 5 healthy controls using microarray. Overall design: LncRNA and mRNA expression profiles of circulating leukocytes from age and sex-matched PD patients and healthy controls were measured by microarray. Each group includes five subjects.

作为一种发病率日益升高的神经退行性疾病，帕金森病（Parkinson's disease, PD）的潜在分子机制仍未明确。长链非编码RNA（long non-coding RNA, lncRNA）在基因表达调控及人类疾病发生发展过程中发挥不可或缺的重要作用。本研究假设长链非编码RNA参与帕金森病的致病进程。我们通过基因芯片（microarray）技术，对5名帕金森病患者与5名健康对照者的循环白细胞中的长链非编码RNA表达谱进行了检测分析。
整体实验设计：采用基因芯片技术检测年龄与性别匹配的帕金森病患者及健康对照者循环白细胞的长链非编码RNA与mRNA表达谱，每组均包含5名受试者。