Global DNA methylation landscape reveals roles of DNA methylation involved in the regulation of drug metabolizing enzyme [II]

In order to elucidate the role of DNA methylation in the DME gene regulation, global DNA methylation and mRNA expression profiles of human tissues and cell lines were examinde by HumanMethylation450 Bead Chip and SurePrint G3 Human Gene Expression 8×60K v2. We demonstrated DNA methylation landscape of the DME genes in human tissues. Although a fraction of DME genes can be regulated by their DNA methylation, the variable DNA methylation status probably affects drug metabolism and response. Bisulphite converted DNA from the 7 samples were hybridized to the Illumina HumanMethylation450 BeadChip.

为阐明DNA甲基化在药物代谢酶（Drug-Metabolizing Enzymes，DME）基因调控中的作用，本研究采用HumanMethylation450 Bead芯片（HumanMethylation450 Bead Chip）与SurePrint G3人类基因表达8×60K v2芯片（SurePrint G3 Human Gene Expression 8×60K v2），对人体组织及细胞系的全基因组DNA甲基化水平与mRNA表达谱开展了检测。本研究明确了人体组织中DME基因的DNA甲基化调控全景图谱。尽管部分DME基因可通过自身DNA甲基化实现转录调控，但DNA甲基化状态的异质性可能会对药物代谢过程及药物响应效果产生影响。研究人员将7份样本的亚硫酸氢盐转化DNA与Illumina HumanMethylation450 Bead芯片（Illumina HumanMethylation450 BeadChip）进行了杂交实验。