H3K9me3 (new lot).OR Head Nuclei

modENCODE_submission_2664 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determe the locations of 125 chromosomal proteins and histone modifications across the Drosophila melanogaster genome. The proteins and modifications under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Keywords: CHIP-chip EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Strain: Oregon-R; Developmental Stage: Mixed Adult; Genotype: wild type; NUMBER OF REPLICATES: 4; EXPERIMENTAL FACTORS: Strain Oregon-R; organism part ; Antibody H3K9me3 (new lot) (target is H3K9me3); Developmental Stage Mixed Adult

modENCODE_submission_2664 本提交源自Gary Karpen主持的modENCODE项目。若需查阅modENCODE项目的完整列表，请访问http://www.genome.gov/26524648。项目目标：本研究旨在定位黑腹果蝇（Drosophila melanogaster）基因组内125种染色体蛋白质与组蛋白修饰位点。所研究的蛋白质及修饰参与DNA复制、基因表达、基因沉默与遗传等核心染色体功能。我们将采用基因组平铺芯片开展染色质免疫沉淀（Chromatin ImmunoPrecipitation, ChIP）实验。初始阶段将使用三种细胞系与两个胚胎发育阶段的染色质进行定位分析，随后将针对部分蛋白质，将分析范围拓展至另外四种动物组织/发育阶段。关于数据使用的条款与条件，请参阅http://www.genome.gov/27528022与http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf。关键词：ChIP-chip 实验类型：ChIP-chip。生物来源：品系：Oregon-R；发育阶段：混合成虫；基因型：野生型；重复次数：4；实验因素：品系Oregon-R；生物体部位；抗体：H3K9me3（新批次）（靶标为H3K9me3）；发育阶段：混合成虫