RNAseq Analysis of wild type and nrt1.13 in normal or low nitrate condition

We report the nitrate-dependent regulation of gene-expression that resulted in the late flowering phenotype. This work studies the impact of NRT1.13 in the nitrate-dependent control of flowering. Wild type and nrt1.13 were grown in hydroponic medium with 2 (normal) or 0.2 (low) mM potasium nitrate. The shoot were harvested at 16 or 25-day after germination for RNA extraction. Three replicates of samples were analyzed.

本研究报道了可导致开花延迟表型的硝酸盐依赖型基因表达调控机制。本研究旨在探讨NRT1.13在硝酸盐依赖型开花调控过程中的作用。将野生型与nrt1.13突变体接种于含有2 mM（常规浓度）或0.2 mM（低浓度）硝酸钾的水培培养基中进行培养，分别于种子萌发后第16天和第25天收取地上组织，用于RNA提取。每组设置三份生物学重复样本进行检测分析。