CCAAT/enhancer-binding protein alpha target genes in myeloid progenitor cells uncovered by chromatin IP. Mus musculus

CCAAT/enhancer-binding protein alpha (C/EBPα) is a lineage-specific transcription factor that directs development of granulocytes. To obtain insights into the genome-wide transcriptome of wild type C/EBPα, we have performed chromatin immunoprecipitation on DNA promoter microarrays (ChIP-chip) in the murine 32D myeloid progenitor cell line. These cells expressed an estradiol-inducible form of wild-type C/EBPα. Overall design: In the murine 32D myeloid progenitor cell line, expressing either an estradiol-inducible form of wild-type C/EBPα. (C/EBPα-ER, n=3, replicates), or as a control ER (n=2, replicates), we have performed chromatin immunoprecipitation on DNA promoter microarrays (ChIP-chip) after 4 hours of estradiol incubation.

CCAAT/增强子结合蛋白α（CCAAT/enhancer-binding protein alpha, C/EBPα）是一种谱系特异性转录因子，可调控粒细胞的发育。为探究野生型C/EBPα的全基因组转录组特征，我们在小鼠32D髓系祖细胞系中开展了基于DNA启动子芯片的染色质免疫共沉淀（chromatin immunoprecipitation on DNA promoter microarrays, ChIP-chip）实验。该细胞系可表达雌二醇诱导型野生型C/EBPα。实验总体设计如下：在该小鼠32D髓系祖细胞系中，我们设置了两组实验样本：一组为表达雌二醇诱导型野生型C/EBPα的细胞（C/EBPα-ER，生物学重复n=3），另一组为仅表达ER空载体的对照细胞（n=2，生物学重复）；两组均在经雌二醇孵育4小时后开展ChIP-chip实验。