PCR screening of miRNA in AIONFH cases and health control

MiRCURY LNATM Universal RT microRNA PCR Panels is utlized to analyze the spectra of miRNAs in serum samples from AIONFH patients and applied the random variance model (RVM) t-test to filter the differentially expressed miRNAs. The distinguishable miRNA expression profiles among AIONFH patients and healthy controls were demonstrated by the results of hierarchical clustering. Initial analysis revealed 10 miRNAs that were differentially expressed. Seven miRNAs (miR-127-3p, miR-1, miR-654-3p, miR-628-3p, miR-432-5p, miR-582-3p, and miR-323a-3p) were found to be significantly downregulated (P<0.01), while three miRNAs (miR-483-5p, miR-483-3p, and miR-885-5p) were markedly upregulated (P<0.01)

本研究采用MiRCURY LNATM通用逆转录微RNA（microRNA, miRNA）PCR检测面板，对股骨头缺血性坏死（AIONFH）患者的血清样本中的miRNA表达谱进行分析，并通过随机方差模型（random variance model, RVM）t检验筛选差异表达miRNA。层次聚类分析结果显示，AIONFH患者与健康对照者的miRNA表达谱存在显著差异。初步分析共筛选出10个差异表达miRNA，其中7个miRNA（miR-127-3p、miR-1、miR-654-3p、miR-628-3p、miR-432-5p、miR-582-3p及miR-323a-3p）显著下调（P<0.01），另有3个miRNA（miR-483-5p、miR-483-3p及miR-885-5p）显著上调（P<0.01）