NURD dependent Reprogramming by Sall4-Jdp2-Esrrb-Glis1 (ChIP-seq). NURD dependent Reprogramming by Sall4-Jdp2-Esrrb-Glis1 (ChIP-seq)

Cell fate decision involves rewiring of the genome, but remains poorly understood at the chromatin level. We report a system to reprogramming somatic cells to pluripotency by four factor combination (Sall4, Esrrb, Jdp2, Glis1). Mechanism study demonstrate that the Sall4-NuRD axis plays a critical role in the early phase of reprogramming. These results identify a previously unrecognized role of NuRD in reprogramming, may help establish early chromatin closing as a pre-requisite step in cell fate control. Overall design: Based on a reprogramming system of 4 factor (WT/N12 Jdp2, Esrrb, Glis1, WT/K5A Sall4), knocking down Gatad2b, Chd4 or dropout single factor.

细胞命运决定涉及基因组的重编程，但在染色质层面的调控机制仍未被充分解析。本研究报道了一套通过四种因子组合（Sall4、Esrrb、Jdp2、Glis1）将体细胞重编程为多能干细胞的实验体系。机制研究表明，Sall4-核小体重塑与去乙酰化复合体（NuRD）轴在重编程的早期阶段发挥关键调控作用。本研究结果揭示了NuRD在重编程过程中此前未被认知的功能，或有助于确立"早期染色质闭合是细胞命运调控的先决步骤"这一核心论点。实验设计概要：基于4因子重编程体系（WT/N12 Jdp2、Esrrb、Glis1、WT/K5A Sall4），分别对Gatad2b、Chd4进行敲低，或进行单因子敲除。