Physicochemical characterization and evaluation of in vitro and in vivo toxicity of goldenberry extract nanoemulsion

ABSTRACT: Oil-in-water (O/W) nanoemulsion containing goldenberry extract was elaborated using a high-energy ultrasonic bath method. Physicochemical characterization of the formulation was carried out by determining pH, mean droplet diameter, polydispersity index (PDI) and zeta potential. Nanoemulsion toxicity was assessed using in vitro assays with tumor and non-tumor cell lines, and in vivo using Caenorhabditis elegans. The pH of the nanoemulsion was 3.84, the mean droplet diameter was 268 ± 7 nm, PDI 0.113 and zeta potential -13.94 mV. Results of the cytotoxicity assays employing non-tumor cells indicated that the extract associated or not with nanoemulsion maintained cell viability at different concentrations tested. In the assays using tumor lineage, it is observed that the nanoemulsion containing the extract had higher antitumor activity than the free extract. As for the in vivo tests, there was no change in the survival rate of the worms.

摘要： 本研究采用高能超声浴法制备了含金浆果（goldenberry）提取物的水包油（O/W）纳米乳液。通过测定pH值、平均液滴直径、多分散性指数（PDI）与Zeta电位，对该制剂开展了理化表征。本研究通过肿瘤细胞系、非肿瘤细胞系体外实验以及秀丽隐杆线虫（Caenorhabditis elegans）体内实验，对该纳米乳液的毒性进行了评估。该纳米乳液的pH值为3.84，平均液滴直径为268±7 nm，多分散性指数（PDI）为0.113，Zeta电位为-13.94 mV。非肿瘤细胞的细胞毒性实验结果显示，无论是否与纳米乳液结合，提取物在各测试浓度下均能维持细胞存活率。在肿瘤细胞系实验中，含提取物的纳米乳液较游离提取物展现出更强的抗肿瘤活性。体内实验结果表明，秀丽隐杆线虫的存活率未发生显著变化。