Temporal and Clonal Progression in Pediatric Ependymoma

Whole genome and exome-based DNA sequencing of germline and tumor specimens from serial samples of a single patient's ependymoma. The findings reveal the extent to which treatment with radiation and chemotherapies resulted in the diversification of the tumor subclonal architecture and shaped the neo-antigen landscape, and provide new insights into possible molecular mechanisms of oncogenesis, treatment response and recurrence.]]>
Sequencing was limited to a single individual, and DNA and RNA from all available biopsies were assayed.]]>

针对单例室管膜瘤（ependymoma）患者的系列样本，对其胚系与肿瘤标本开展全基因组及外显子组DNA测序。本研究结果揭示了放疗与化疗可在多大程度上推动肿瘤亚克隆结构的多样化进程，并重塑新抗原（neo-antigen）谱，同时为肿瘤发生、治疗应答及复发的潜在分子机制提供了全新见解。
本次测序仅针对单一个体开展，且对所有可用活检样本中的DNA与RNA均进行了检测分析。