Expression data from classical dendritic cells isolated from mouse lymph nodes

Dendritic cells increase expression of pro-inflammatory cytokines and chemokines and undergo metabolic reprogramming upon TLR activation. Mice fed conventional diet (CD) or 60% high-fat diet (HFD) were treated by skin application of imiquimod (IMQ) during 6 days and classical dendritic cells (cDC) were isolated from inguinal lymph nodes (iLN) to reveal inflammatory and metabolic pathways that are sensitive to TLR7/8 activation and a high fatty acid environment. We used microarray to identify pathways in classical dendritic cells from IMQ-treated mice that link inflammation and metabolism in a high fatty acid environment. cDC were isolated from skin-draining iLN from IMQ-treated mice. Mice were fed CD or 60% HFD and belly skin was treated with a daily dose of cream (62.5 mg) containing 5% IMQ during 6 days. CD3−CD19−CD64−Ly6G−CD11c+MHCII+ cDC were sorted from mouse iLN using BD Influx Cell Sorter (BD Biosciences) and used for RNA isolation.

树突状细胞（Dendritic cells）经Toll样受体（TLR）激活后，可上调促炎细胞因子与趋化因子的表达，并发生代谢重编程。本研究将喂食常规饮食（conventional diet, CD）或60%高脂饮食（high-fat diet, HFD）的小鼠，通过皮肤涂抹咪喹莫特（imiquimod, IMQ）连续处理6天，随后从腹股沟淋巴结（inguinal lymph nodes, iLN）中分离经典树突状细胞（classical dendritic cells, cDC），旨在揭示对TLR7/8激活及高脂肪酸环境敏感的炎症与代谢通路。我们采用基因芯片（microarray）技术，在经IMQ处理的小鼠的经典树突状细胞中，鉴定出高脂肪酸环境下连接炎症与代谢的相关通路。经典树突状细胞（cDC）取自经IMQ处理小鼠的皮肤引流腹股沟淋巴结（iLN）。实验中小鼠被喂食常规饮食（CD）或60%高脂饮食（HFD），腹部皮肤每日涂抹含5%咪喹莫特（IMQ）的乳膏（剂量为62.5 mg），连续处理6天。通过BD Influx细胞分选仪（BD Biosciences）从小鼠腹股沟淋巴结（iLN）中分选出CD3⁻CD19⁻CD64⁻Ly6G⁻CD11c⁺MHCII⁺的经典树突状细胞（cDC），并将其用于RNA提取。