Transcriptional analysis of kidney infiltrating T cells after ischemia reperfusion injury reveals a pathophysiologic role for the chemokine (C-C motif) receptor 5

We examined the transcriptional responses in kidney infiltrating T cells 6 hrs after IRI CD3 (+) T cells were purified applying magnetic beads to mononuclear cells extracted from kidney by percoll gradient methods. The total T-cell RNA was isolated, and trabscript abundance measured with array-based PCR

本研究探究了缺血再灌注损伤（Ischemia-Reperfusion Injury, IRI）发生6小时后肾脏浸润T细胞的转录应答反应。研究人员首先通过Percoll密度梯度离心法（Percoll gradient）从肾脏组织中提取单个核细胞，再通过磁珠分选法纯化得到CD3阳性T细胞（CD3(+) T cells）；随后提取总T细胞RNA，并利用阵列PCR（array-based PCR）检测转录本的丰度。