Genetically Barcoded SupT1 Cells

Genetic barcoding of non-adherent mammalian cells utilizing retroviral technology enahnces the power of mulitplexed applications. SupT1 cells can be separated based on fluorescence intensity, and are stable for long periods of time.
Conclusion:
The growing number of existing fluorescent proteins and derivates with distinct absorbance/emission spectra, combined with the growing number of affordable detection devices and lasers, increases the versatility of multiplexing, making fluorescent genetic barcoding a powerful tool for flow cytometry-based analysis. Each figure includes cells with no fluorescent protein as a negative control allowing for gating.

利用逆转录病毒技术对非贴壁哺乳动物细胞进行遗传条形码标记，可增强多重应用的效力。SupT1细胞可依据荧光强度进行分选，且能够长期保持稳定。
结论：
现有具备独特吸收/发射光谱的荧光蛋白及其衍生物数量持续增加，加之低成本检测设备与激光器日益普及，进一步提升了多重检测的通用性，使得荧光遗传条形码标记成为基于流式细胞术分析的强力工具。每张配图均设置未表达荧光蛋白的细胞作为阴性对照，用于设门操作。