Blood transcripts from female Japanese quail given LPS injection or saline. Blood transcripts from female Japanese quail given LPS injection or saline

The aim of this experiment was to test how female Japanese quail respond to being housed with sick (LPS treated) relative to control males. LPS injected females were also tested. Overall design: Adult female Japanese quail received either saline or LPS injection. All animals were allowed to interact with males for 3 h. Half of the saline injected birds were exposed to LPS injected males and the other half to control males. A blood sample was collected from the brachial vein at the end of the 3h. Blood was spun down and plasma and red blood were separated. RNA was extracted from red blood portion.

本实验旨在探究日本鹌鹑雌性个体与经脂多糖（LPS）处理的患病雄性个体同笼饲养时的响应模式，并以对照雄性组作为参照；同时也对经LPS处理的雌性个体开展了相关测试。
实验设计概述：成年雌性日本鹌鹑分别接受生理盐水或LPS注射。所有受试动物均与雄性个体进行3小时的互动。其中半数经生理盐水注射的雌性个体暴露于LPS处理的雄性个体，另一半则与对照雄性个体接触。在3小时互动结束后，从肱静脉采集血液样本。血液经离心处理后分离得到血浆与红细胞组分，随后从红细胞组分中提取RNA。