Data from: "NGS based generation of expressed sequence tags for Lymantria dispar and Lymantria monacha, two closely related lepidopteran species with different responses to parasitism by Glyptapanteles liparidis" in Genomic Resources Notes accepted 1 December 2013 to 31 January 2014

Introduction: The gypsy moth, Lymantria dispar, and the nun moth, Lymantria monacha, are closely related species (Lepidoptera, Lymantriidae), co-seasonal and economically important forest pests on broadleaf and coniferous trees. In Central Europe, gypsy moth larvae are frequently parasitized by the gregarious, endoparasitic wasp Glyptapanteles liparidis (Hymenoptera, Braconidae). At oviposition, the female wasp injects between 10 and up to 100 eggs into the hemocoel of a single host larva, together with venom and calyx fluid containing polydnavirus (PDV) particles that subsequently play a critical role in suppressing the host immune response so that successful development of the parasitoid can proceed (Schopf 2007). These viruses, which are integrated in the genomic DNA of the wasp and undergo replication only in the female’s ovary, rapidly enter host hemocytes, fat body, and nervous system following parasitization, and viral genes are expressed. In L. dispar larvae parasitized by G. liparidis, the host’s hemocytes alter their behavior, fail to spread properly (thereby inhibiting the encapsulation response) and partly undergo programmed cell death (apoptosis), resulting in a dramatic drop in the host’s total hemocyte number (Schafellner and Schläger 2009).

引言：舞毒蛾（Lymantria dispar）与茸毒蛾（Lymantria monacha）为亲缘关系密切的物种，隶属于鳞翅目（Lepidoptera）毒蛾科（Lymantriidae），二者发生期同步，均为危害阔叶树与针叶树的重要经济林业害虫。在中欧地区，舞毒蛾幼虫常会被群居性内寄生蜂——舞毒蛾绒茧蜂（Glyptapanteles liparidis，膜翅目Hymenoptera，茧蜂科Braconidae）寄生。该雌蜂产卵时，会向单头寄主幼虫的血腔内注入10至最多100枚卵，同时注入毒液以及含有多DNA病毒（polydnavirus, PDV）颗粒的萼液；此类病毒颗粒后续会在抑制寄主免疫反应中发挥关键作用，保障寄生蜂顺利完成发育（Schopf 2007）。这类病毒整合于寄生蜂的基因组DNA中，仅在雌蜂卵巢内进行复制；寄生后，病毒会快速侵入寄主的血细胞、脂肪体与神经系统，并表达病毒基因。在被舞毒蛾绒茧蜂寄生的舞毒蛾幼虫体内，寄主血细胞会出现行为异常，无法正常延展，进而抑制包囊反应，部分血细胞还会发生程序性细胞死亡（凋亡），最终导致寄主总血细胞数量大幅下降（Schafellner and Schläger 2009）