Multi-omics association reveals the effects of intestinal microbiome-host interactions on fat deposition in broiler lines divergently selected for abdominal fat content

The lean and fat broilers were derived from the 21st generation of the Northeast Agricultural University broiler lines divergently selected for abdominal fat content (NEAUHLF). In brief, broilers in the two lines (lean line and fat line) of the NEAUHLF have the same Arbor Acres ancestry, but were divergently selected by the concentration of very low-density lipoprotein and abdominal fat percentage (AFP) at 7 weeks old. After 21 generations of selection, there is over a 10-fold difference in abdominal fat percentage between the two lines, representing a classical obese-lean-study model. At age of 7 weeks, ten male individuals of each line were selected randomly to investigate the interactions between gut microbiome, host gene expression and obesity. Overall design: All broilers were raised in similar feeding surroundings and had free access to feed and water. At age of 7 weeks, ten male individuals of each line were selected randomly to investigate the interactions between gut microbiome, host gene expression and obesity.

低脂肉鸡与高脂肉鸡均来源于东北农业大学腹脂含量双向选择肉鸡品系（NEAUHLF）的第21世代群体。简言之，NEAUHLF的两个品系（低脂品系与高脂品系）的肉鸡均拥有相同的爱拔益加（Arbor Acres）血统，但分别以7周龄时的极低密度脂蛋白浓度与腹脂率（abdominal fat percentage, AFP）为指标进行双向选育。经过21世代选育后，两个品系的腹脂率差异超过10倍，是经典的肥胖-低脂研究模型。7周龄时，研究人员从每个品系中随机选取10只公鸡，以探究肠道微生物组、宿主基因表达与肥胖之间的互作关系。实验整体设计如下：所有肉鸡均在一致的饲养环境中培育，且可自由采食与饮水；7周龄时，从每个品系中随机选取10只公鸡，以探究肠道微生物组、宿主基因表达与肥胖之间的互作关系。