Effect of cisplatin on gene expression of human microvascular endothelial cells

Cisplatin (DDP) is a widely used chemotherapeutic agent that induces apoptosis in various cancer cells, but its effects on endothelial cells such as HMEC-1 (Human Microvascular Endothelial Cell line-1) are less well understood. This study aims to explore the transcriptional response of HMEC-1 to cisplatin treatment. RNA-seq analysis was performed to identify differentially expressed genes following cisplatin stimulation, providing insights into the molecular mechanisms underlying the cellular response to chemotherapy in endothelial cells. The results highlight key pathways and genes involved in the stress response, apoptosis, and cellular survival in HMEC-1 cells upon cisplatin treatment. To investigate the effect of cisplatin on HMEC-1 cells, we treated the cells with cisplatin at a specific concentration for a defined period. RNA was extracted and RNA-seq was performed on treated and control cells to profile the gene expression changes induced by cisplatin. Comparative gene expression profiling analysis was conducted to identify differentially expressed genes between cisplatin-treated and control HMEC-1 cells, focusing on pathways related to apoptosis, stress response, and cellular survival.

顺铂（Cisplatin，DDP）是一种临床应用广泛的化疗药物，可诱导多种癌细胞发生凋亡，但其对HMEC-1（人微血管内皮细胞系-1）等内皮细胞的作用效果尚缺乏深入阐释。本研究旨在探究HMEC-1细胞对顺铂处理的转录应答。我们通过RNA-seq（RNA测序）分析顺铂刺激后的差异表达基因，以期揭示内皮细胞响应化疗药物的潜在分子机制。研究结果明确了顺铂处理后HMEC-1细胞中参与应激反应、凋亡及细胞存活的关键通路与功能基因。为探究顺铂对HMEC-1细胞的影响，本研究以特定浓度的顺铂处理细胞并设置固定孵育时长；随后分别提取顺铂处理组与对照组细胞的总RNA，开展RNA-seq测序以刻画顺铂诱导的基因表达变化谱。通过比较两组的基因表达谱，筛选出顺铂处理组与对照组HMEC-1细胞间的差异表达基因，并重点聚焦于与凋亡、应激反应及细胞存活相关的信号通路。