Nucleotide-resolution, genome-wide, 5'-end maps of the transcriptome of Propionibacterium acnes (cultured with and without potassium downshift). Cutibacterium acnes KPA171202

The differential RNA-seq data contained within this entry is complemented by global RNA-seq and microarray data, which is also deposited in GEO. Overall design: Duplicate cultures of Propionibacterium acnes strain KPA171202 were grown exponentially in batch culture under anaerobic growth. Samples were taken following subculture with and without potassium downshift (i.e. removal from medium). This produced 4 samples; 2 replicates x 2 conditions. Aliquots of each of the 4 samples were then incubated with or without incubation TAP (tobacco acid pyrophosphatase) before library construction. Thus, 8 libraries were analysed. TAP treatment allows the cloning and sequencing of 5' ends that were originally triphosphorylated.

本条目所收录的差异RNA测序（differential RNA-seq）数据，辅以全局RNA测序（global RNA-seq）与微阵列数据，此类数据亦已提交至基因表达综合数据库（Gene Expression Omnibus，简称GEO）。
实验整体设计如下：将痤疮丙酸杆菌（Propionibacterium acnes）KPA171202菌株的重复培养物置于厌氧条件下的分批培养体系中进行指数生长期培养。在传代培养后，分别施加钾离子下调处理（即从培养基中移除钾离子）与不施加该处理，随后采集样本，由此得到4组样本：2次生物学重复 × 2种实验条件。将4组样本的等分试样分别进行烟草酸焦磷酸酶（tobacco acid pyrophosphatase，简称TAP）孵育处理与未进行该处理，随后进行文库构建。由此共计得到8个待分析的测序文库。TAP处理可实现原本带有三磷酸基团的5'端序列的克隆与测序。