HSFA1a controls plant heat stress response through its action on the 3D chromatin reorganization of enhancer-promoter interactions [DAP-seq]

We investigated the role of HSFA1a, a master regulator of heat stress response, in this reorganization through promotion of the formation of promoter/enhancer chromatin loops. We performed Amplified DNA affinity purification sequencing assay to determine HSFA1a binding sites. Comparative analysis of DNA affinity profiling of HSFA1a-Halo tag with control Halo-tag.

本研究聚焦热胁迫响应主调控因子HSFA1a，探究其在染色质重塑过程中的作用机制——通过促进启动子/增强子染色质环的形成。我们采用扩增DNA亲和纯化测序（Amplified DNA affinity purification sequencing）实验明确了HSFA1a的结合位点，并开展了HSFA1a-Halo标签与对照Halo标签的DNA亲和谱对比分析。