Additional file 1: Table S1. of Reversing chromatin accessibility differences that distinguish homologous mitotic metaphase chromosomes

Human lymphoblastoid cell lines harvested for metaphase chromosomes were hybridized with single copy (sc) probes from indicated GRCh37 genomic coordinates. Concentrations of each treatment, optimized for in situ hybridization are indicated. Only cells treated with 5 μM of UNC1999 from GM10958 cell line were included in the statistical analysis of DA for SNRPN and ADORA2B, due to an insufficient number of mitoses at 15 μM. The numbers of metaphase cells with and without (w/o) DA are indicated for each treatment dose along with corresponding no treatment control prepared at the same time.

为制备中期染色体而收获的人类淋巴母细胞系，与来自指定GRCh37基因组坐标的单拷贝（single copy, sc）探针进行杂交。针对原位杂交（in situ hybridization）优化后的各处理组浓度均已标注。鉴于15 μM浓度下有丝分裂相细胞数量不足，仅将GM10958细胞系中经5 μM UNC1999处理的细胞纳入SNRPN与ADORA2B基因DA的统计学分析。各处理浓度组及同期制备的未处理对照组中，存在DA与不存在DA（w/o）的中期细胞数量均已标注。