AR and ERG ChIP-seq in presence or absence of PRMT5. Homo sapiens

Replicates of ChIP seq data using AR and ERG antibodies from VCaP cell lines harboring inducible shRNA constructs for PRMT5 (3 independent, sh1, sh2, sh3) and 1 non targeting control (NTC), all sample stimulated with ligand, either DHT or R1881 Overall design: AR ChIPseq in VCaP cells, 3 replicates each of DHT stimulated cells with one of 4 inducible shRNA constructs, sh1, sh2, sh3 targeting PRMT5 or NTC non targeting controls. Furthermore 2 AR ChIPseq in the same system stimulated with R1881 for targeting constructs sh1, sh2 and NTC. ERG ChIPseq 2 replicates DHT stimulated, each of sh1, sh2, sh3 constructs. Cells where induced (doxycyclin) for shRNA expression on day0, hormone starved on day3 and harvested on day5.

本数据集为使用雄激素受体（Androgen Receptor, AR）与ETS相关基因（ETS-related gene, ERG）抗体，针对携带蛋白质精氨酸甲基转移酶5（Protein Arginine Methyltransferase 5, PRMT5）诱导型短发夹RNA（short hairpin RNA, shRNA）构建体的VCaP细胞系所获染色质免疫共沉淀测序（ChIP-seq）生物学重复样本：其中包含3组独立重复样本（sh1、sh2、sh3）以及1组非靶向对照（Non-Targeting Control, NTC），所有样本均经配体刺激，刺激剂为二氢睾酮（Dihydrotestosterone, DHT）或甲酰勃龙（R1881）。

实验总体设计如下：在VCaP细胞中开展AR ChIP-seq实验，靶向PRMT5的sh1、sh2、sh3与非靶向对照NTC这4种诱导型shRNA构建体对应的二氢睾酮刺激细胞样本，每组均设置3次生物学重复。此外，在相同细胞体系中，针对靶向构建体sh1、sh2及非靶向对照NTC，设置了经甲酰勃龙R1881刺激的AR ChIP-seq实验，每组各2次生物学重复。ERG ChIP-seq实验则包含靶向构建体sh1、sh2、sh3对应的二氢睾酮刺激样本，每组均设置2次生物学重复。

所有细胞均于第0日经多西环素（Doxycycline）诱导shRNA表达，第3日进行激素剥夺处理，并于第5日收集细胞。