SRSF1 knockdown in HEK293T cells with ionizing radiation

The goal of the study is to identify differentially expressed isoforms in response to SRSF1 knockdown and/or ionizing radiation in HEK293T cells Overall design: There are four conditions: siSRSF1 + 10 Gy ionizing radiation (IR), siControl + 10 Gy IR, siSRSF1 + 0 Gy IR, siControl + 0 Gy IR. siSRSF1 means SRSF1 was knocked down using an siRNA oligo targeting all known isoforms of SRSF1. siControl means the cells were transfected with a pool of non-targeting oligos as control. 10 Gy means cells were exposed to 10 Gy of IR, while 0 Gy are mock-trated samples. There are five biological replicates of each condition. The samples were collected 48 hours after IR or mock IR.

本研究旨在鉴定HEK293T细胞中响应SRSF1敲低和/或电离辐射的差异表达转录本异构体（isoforms）。
实验设计：共设置4组处理条件：siSRSF1+10 Gy电离辐射（IR）组、siControl+10 Gy IR组、siSRSF1+0 Gy IR组、siControl+0 Gy IR组。其中，siSRSF1指使用靶向SRSF1所有已知转录本异构体的小干扰RNA（siRNA）寡核苷酸对细胞进行SRSF1敲低；siControl指细胞转染非靶向寡核苷酸混合物作为空白对照；10 Gy组指细胞接受10 Gy剂量的电离辐射处理，0 Gy组为模拟辐射处理的对照样本。每组处理设置5个生物学重复，所有样本均于辐射或模拟辐射处理后48小时收集。