Underlying data of Figure 2. Representative cases of the analysis of upstream transcription factors using TFEL against specific promoters.

Underlying data of Figure 2 of "Genome-wide screening of upstream transcription factors using an expression library."
(A-C) Representative cases of the
analysis of upstream transcription factors using TFEL against specific
promoters: (A) vascular endothelial growth factor (gene name: Vegfa), (B) fatty acid synthase (gene name: Fasn), and (C) sterol regulatory element-binding protein-1c (gene name: Srebf1c).
The first screening data performed using a 20-clone pool assay are shown (for C: Srebf1c, 10-clone pools were used). Individual clones were identified in the second screening. (D) Representative case identifying the combinatorial interactions on a specific promoter. In this assay, we screened for transcription factor(s) that suppress LXR activity on the SREBP-1c promoter (-249 to -144). To activate LXR and to improve the sensitivity of the assay, we added an LXR ligand T0901317 to the culture media.

本数据集为论文《基于表达文库的上游转录因子全基因组筛选》（Genome-wide screening of upstream transcription factors using an expression library）中图2的原始数据。
(A-C) 采用转录因子表达文库（TFEL）针对特定启动子开展上游转录因子分析的典型案例：(A) 血管内皮生长因子（vascular endothelial growth factor，基因名：Vegfa）、(B) 脂肪酸合酶（fatty acid synthase，基因名：Fasn）以及(C) 固醇调节元件结合蛋白-1c（sterol regulatory element-binding protein-1c，基因名：Srebf1c）。
本部分展示首次筛选的实验数据，实验采用20克隆混合池检测方案；针对C组的Srebf1c，实验采用10克隆混合池方案。第二轮筛选中完成了单克隆的鉴定。
(D) 特定启动子上组合式调控互作鉴定的典型案例。本实验针对SREBP-1c启动子区域（-249至-144）上可抑制肝X受体（liver X receptor, LXR）活性的转录因子进行筛选。为激活肝X受体并提升实验灵敏度，我们向培养基中添加了肝X受体配体T0901317。