Gene expression-based dentification of microRNA-9-3 target genes in breast epithelial cells

In current study, DES could effectively suppress the expression of miR-9-3 through the ERa-dependent epigenetic machinery in MDECs and MCF-7 cells. We want to figure out the biological significance and the potential targets of miR-9-3 and the related signaling cascades. MCF-7 cells were transfected with two pre-designed maturated mimics of miR-9-3, miR-9 and miR-9*. After 48hr of transfection, total RNA was collected for gene expression analysis to figure out the potential targets of miR-9-3.

本研究中，己烯雌酚（DES）可通过依赖于雌激素受体α（ERα）的表观遗传调控机制，有效抑制MDECs与MCF-7细胞中微小RNA-9-3（miR-9-3）的表达。本研究旨在阐明miR-9-3的生物学意义、潜在靶标及其相关信号级联通路。我们将两种预设计的miR-9-3成熟模拟物——miR-9与miR-9*——转染至MCF-7细胞中。转染48小时后，收集总RNA用于基因表达分析，以明确miR-9-3的潜在靶标。