Consumption by house flies FL data sheet.

Granular fly baits remain one of the most popular and effective forms of chemical control of house flies (Musca domestica). While these baits contain a sucrose phagostimulant, some also contain the bittering agent denatonium benzoate at 20 or 100 ppm, as a feeding deterrent for humans. The response of adult house flies to 10, 100 and, 1000 ppm denatonium benzoate in sucrose solution was compared to response to sucrose only solution using proboscis extension response and no-choice consumption assays. Three house fly strains, DBQB, WD, and UF, each from a different Florida dairy farm, were tested within two generations of collection. Strain DBQB, but not strains WD or UF, had a known history of exposure to toxic baits containing denatonium benzoate, although recent insecticide history was unknown. For males, all strains avoided proboscis extension response and consumption with 1000 ppm, but not with 10 ppm. Males of WD and UF strains, but not DBQB strain showed significantly lower frequency of proboscis extension response at 100 ppm. Males of WD strain, but not DBQB or UF strains, also avoided consuming 100 ppm. For females, the pattern of significance for proboscis extension response was the same for all strains: avoidance for 1000 ppm but not for 10 or 100 ppm. Female consumption was significantly reduced at 1000 ppm for the DBQB and WD strains, but not for the UF strain, but no strain avoided 10 or 100 ppm for consumption. Thus, our results suggest that in some populations of house flies, 100 ppm of denatonium benzoate may reduce consumption by males, although not consumption by females.

颗粒型杀蝇饵剂仍是防治家蝇（Musca domestica）最常用且高效的化学防控手段之一。此类饵剂通常以蔗糖作为取食刺激剂，部分产品还会添加浓度为20或100ppm的苯甲地那铵（denatonium benzoate）作为人类拒食剂。本研究通过喙伸长反应（proboscis extension response）与无选择取食实验，比较了成年家蝇对蔗糖溶液中添加10、100及1000ppm苯甲地那铵的行为反应，并以纯蔗糖溶液处理组作为对照。
实验共使用3株家蝇品系——DBQB、WD及UF，均采自美国佛罗里达州不同的奶牛场，且均在采集后的两代内完成测试。其中DBQB品系具有接触含苯甲地那铵毒饵的已知历史，而WD与UF品系无此记录，但三者近期的杀虫剂接触史均未明确。
对于雄性家蝇，所有品系在1000ppm浓度下均表现出喙伸长反应与取食行为的回避，但在10ppm浓度下无此现象；WD和UF品系的雄性家蝇在100ppm浓度下的喙伸长反应频率显著降低，而DBQB品系则无此变化；仅WD品系的雄性家蝇会回避100ppm浓度下的取食行为，DBQB与UF品系无此表现。
对于雌性家蝇，所有品系的喙伸长反应显著性变化模式均一致：仅在1000ppm浓度下表现出回避行为，而10与100ppm浓度下无此现象。在取食量方面，DBQB与WD品系的雌性家蝇在1000ppm浓度下的取食量显著降低，而UF品系无此变化；且所有品系的雌性家蝇在10与100ppm浓度下均未出现取食回避行为。
综上，本研究结果表明，在部分家蝇种群中，100ppm浓度的苯甲地那铵可抑制雄性家蝇的取食行为，但对雌性家蝇的取食无显著影响。