spt16 Mutant alleles that activate cryptic promoters in dominant fashion (1).
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(1)All mutant alleles listed here, on low-copy plasmids, activate the reporter genes his4-912δ, lys2-128δ, and GAL1pr-FLO8-HIS3 at 30°C in SPT16 cells (strains AW11-9a and FY2393). Some data for spt16-E857K, spt16-E763G, spt16-312, and spt16-319 are from [42].(2)SPT16 cells: strains AW11-9a and FY2393; spt16Δ cells: strain KanB-[plasmid].(3)Not dissected further.(4)Not determined.(5)Not applicable.
(1) 本文所列全部突变等位基因均搭载于低拷贝质粒(low-copy plasmids)上,可在30℃条件下于SPT16细胞(菌株AW11-9a与FY2393)中激活报告基因(reporter genes)his4-912δ、lys2-128δ及GAL1pr-FLO8-HIS3。针对spt16-E857K、spt16-E763G、spt16-312及spt16-319的部分数据源自文献[42]。
(2) SPT16细胞对应菌株为AW11-9a与FY2393;spt16Δ细胞对应菌株为KanB-[质粒]。
(3) 未进行进一步拆解分析。
(4) 尚未测定。
(5) 不适用。
创建时间:
2015-12-02



