Four differentially expressed genes involved in the synthesis of flavan 3 ols in the testa of contrasting colored seeds of Chilean landraces of Phaseolus vulgaris L

The study focuses on the genetic and biochemical characteristics of Chilean common bean landraces, particularly 'Peumo' and 'Tortola'. It highlights that 'Peumo' has a significantly higher content of condensed tannins, such as catechin and epicatechin, during reproductive development under deficit irrigation compared to 'Tortola'. Through RNA sequencing, the research identifies four differentially expressed genes (DEGs) linked to the production and accumulation of flavan-3-ols in the testa of these landraces. Additionally, the study analyzes 962 and 483 DEGs at different developmental stages in both landraces. A key gene, Phvul.002G079300, is identified and validated as influential in the reduced production and transport of catechin and epicatechin in 'Tortola'. This research is significant in understanding the genetic factors influencing seed testa color and may guide future molecular breeding and biochemical studies to enhance the color and antioxidant properties of common beans.

本研究聚焦智利普通菜豆地方品种的遗传与生化特性，重点针对‘Peumo’与‘Tortola’两个地方品种展开。研究表明，与‘Tortola’相比，‘Peumo’在亏缺灌溉条件下的生殖发育阶段中，缩合单宁(condensed tannins)、儿茶素(catechin)与表儿茶素(epicatechin)的含量显著更高。通过RNA测序，本研究在这两个地方品种的种皮(testa)中鉴定出4个与黄烷-3-醇(flavan-3-ols)合成与积累相关的差异表达基因(DEGs)。此外，本研究还分析了两个地方品种在不同发育阶段的962个和483个差异表达基因。研究鉴定并验证了关键基因Phvul.002G079300，该基因对‘Tortola’中儿茶素与表儿茶素的合成与转运受限具有显著调控作用。本研究对于解析影响菜豆种皮颜色的遗传因子具有重要意义，可为未来通过分子育种与生化研究提升普通菜豆的种皮色泽与抗氧化特性提供指导。