series5_GSE147507 : Differential gene expression of A549 Cells_ SARS-CoV-2 (MOI 2.0) infected vs. Mock control

Differentially expressed genes between SARS-Cov-2 (MOI 2.0) infected vs Mock control from A549 cells (series5_GSE147507) with fold change, raw counts, and normalized counts for each gene. <br>RNA-Seq reads (FASTQ) of biological triplicates from A549 cells, mock treated or infected with SARS-CoV-2 (USA-WA1/2020) were downloaded from NCBI SRA and data analysis was performed using CLC Genomics workbench (CLC v20). The reads were aligned to the human genome (hg38) with annotations (Ensemble v91) and then quantification and differential expression of genes between SARS-Cov-2 infected vs. mock control samples was performed. The differentially expressed genes were filtered by FDR P-value&lt;0.05 and uploaded here. <br><br><br><br><br><br><br><br><br><br><br> <br>

本数据集包含来自A549细胞（数据集系列：series5_GSE147507）中以感染复数（MOI）2.0感染严重急性呼吸综合征冠状病毒2型（SARS-Cov-2）与Mock对照组之间的差异表达基因（differentially expressed genes），涵盖每个基因的倍数变化（fold change）、原始计数（raw counts）与标准化计数（normalized counts）信息。
研究团队从美国国家生物技术信息中心序列读取档案（NCBI SRA）下载了经Mock处理或感染SARS-CoV-2（USA-WA1/2020）的A549细胞的三次生物学重复的RNA测序（RNA-Seq）读数（FASTQ格式），并使用CLC基因组工作台（CLC Genomics workbench，CLC v20）开展数据分析。将测序读数比对至带有注释（Ensemble v91）的人类基因组（hg38），随后完成SARS-Cov-2感染组与Mock对照组样本间的基因定量与差异表达分析。本数据集通过错误发现率P值（FDR P-value）<0.05的筛选阈值，得到符合要求的差异表达基因并上传至此。