Table_5_Genome-Wide Identification and Analysis of the NF-Y Gene Family in Potato (Solanum tuberosum L.).xlsx

Nuclear factor Y (NF-Y) is a ubiquitous transcription factor in eukaryotes, which is composed of three subunits (NF-YA, NF-YB, and NF-YC). NF-Y has been identified as a key regulator of multiple pathways in plants. Although the NF-Y gene family has been identified in many plants, it has not been reported in potato (Solanum tuberosum). In the present study, a total of 41 NF-Y proteins in potato (StNF-Ys) were identified, including 10 StNF-YA, 22 StNF-YB, and nine StNF-YC subunits, and their distribution on chromosomes, gene structure, and conserved motif was analyzed. A synteny analysis indicated that 14 and 38 pairs of StNF-Y genes were orthologous to Arabidopsis and tomato (Solanum lycopersicum), respectively, and these gene pairs evolved under strong purifying selection. In addition, we analyzed the expression profiles of NF-Y genes in different tissues of double haploid (DM) potato, as well as under abiotic stresses and hormone treatments by RNA-seq downloaded from the Potato Genome Sequencing Consortium (PGSC) database. Furthermore, we performed RNA-seq on white, red, and purple tuber skin and flesh of three potato cultivars at the tuber maturation stage to identify genes that might be involved in anthocyanin biosynthesis. These results provide valuable information for improved understanding of StNF-Y gene family and further functional analysis of StNF-Y genes in fruit development, abiotic stress tolerance, and anthocyanin biosynthesis in potato.

核因子Y（Nuclear factor Y, NF-Y）是真核生物中普遍存在的转录因子，由NF-YA、NF-YB与NF-YC三个亚基构成。NF-Y已被鉴定为植物多条生物学通路的关键调控因子。尽管诸多植物的NF-Y基因家族已被成功解析，但目前尚未见马铃薯（Solanum tuberosum）中相关研究的报道。本研究共鉴定出41个马铃薯NF-Y蛋白（StNF-Ys），包含10个StNF-YA亚基、22个StNF-YB亚基与9个StNF-YC亚基，并对其染色体分布、基因结构及保守基序展开了分析。共线性分析结果显示，分别有14对与38对StNF-Y基因与拟南芥（Arabidopsis）、番茄（Solanum lycopersicum）存在直系同源关系，且这些基因对均经历了强烈的纯化选择作用。此外，本研究依托从马铃薯基因组测序联盟（Potato Genome Sequencing Consortium, PGSC）数据库下载的RNA-seq数据，分析了双单倍体（double haploid, DM）马铃薯不同组织以及非生物胁迫与激素处理条件下NF-Y基因的表达谱。进一步地，本研究针对3个马铃薯品种块茎成熟阶段的白色、红色与紫色块茎表皮及果肉组织开展了RNA-seq测序，以筛选可能参与花青素生物合成的候选基因。上述研究结果可为深入解析马铃薯StNF-Y基因家族，以及进一步开展StNF-Y基因在马铃薯果实发育、非生物胁迫耐受与花青素生物合成过程中的功能研究提供重要参考依据。