Genome sequencing and assembly of G. herbaceum and G. arboreum using long PacBio reads

we sequenced and assembled only two extant diploid A-genomes G. herbaceum (A1) and G. arboreum (A2) by integrating high-coverage SMRT long-read sequencing, paired-end sequencing and Hi-C technologies. Our initial assembly of G. herbaceum is of high quality to enable both coarse and fine-scale comparative cotton genomic studies. The quality of G. arboreum assembly in this report using high-depth coverage of long reads have significant improvement than all previously released assemblies . Our work makes an important step towards unraveling cotton genome evolution, and provides the valuable genomic resource to facilitate genetic improvement of cotton. If you use these data, please cite the paper: Huang G. et al. Genome sequence of Gossypium herbaceum, and genome update of Gossypium arboreum and Gossypium hirsutum provide insights into cotton A-genome evolution. Nature Genetics, 2020.

本研究整合高覆盖度SMRT长读长测序（SMRT long-read sequencing）、双端测序（paired-end sequencing）与Hi-C技术，仅对两个现存二倍体A基因组棉属物种——草棉（Gossypium herbaceum, A1）与亚洲棉（Gossypium arboreum, A2）完成了测序与组装。我们对草棉的初始组装质量优异，可支撑棉属基因组的粗粒度与细粒度比较研究。本研究中采用高深度长读长测序数据构建的亚洲棉组装结果，其质量较此前所有已发布的同类组装均有显著提升。本研究为解析棉属基因组演化历程迈出了重要一步，同时为推动棉花遗传改良提供了宝贵的基因组学资源。若使用本数据集，请引用以下论文：Huang G. 等人. 《草棉基因组序列解析及亚洲棉、陆地棉基因组更新为棉花A基因组演化研究提供新见解》, 《自然·遗传学》, 2020.