EEL mouse sagittal atlas 168 genes RNA spatial data

<strong>Raw RNA locations of the mouse atlas produced by EEL FISH for 168 genes.</strong> <br> RNA files are in the .parquet format which can be opened with FISHscale (https://github.com/linnarsson-lab/FISHscale) or any other parquet file reader (https://arrow.apache.org/docs/index.html) <br> <strong>RNA .parquet files</strong> Seven sagittal sections of the mouse brain with 168 detected genes, sampled at the medial-lateral positions of -140 µm, 600 µm, 1200 µm, 1810 µm, 2420 µm, 3000 µm and 3600 µm measured from the midline. <br> Position and gene label for all RNA molecules. <br> "c_px_microscope_stitched" contains X coordinates. "r_px_microscope_stitched" contians Y coordinates. The unit are pixels with a size of 0.18 micrometer. Multiply by 0.18 to get um scale. <br> <strong>Tissue polygons .csv files</strong> CSV files demarking the sample borders for the 7 mouse atlas sections. -140 µm, 600 µm, 1200 µm, 1810 µm, 2420 µm, 3000 µm, 3600 µm Some RNA molecules are detected outside the tissue area. To remove these you can open the dataset in FISHscale and give it the polygons in the .csv files. If you do not want to use FISHscale, you can use this code to find which points are inside the tissue: https://github.com/linnarsson-lab/FISHscale/blob/master/FISHscale/utils/inside_polygon.py <br> <strong>Gene colors .pkl file</strong> Pickled Python dictionary with gene colors used in the paper for the mouse atlas.

**由EEL FISH生成的、针对168个基因的小鼠图谱原始RNA定位数据**
RNA数据文件采用Parquet格式，可通过FISHscale（https://github.com/linnarsson-lab/FISHscale）或任意Parquet文件阅读器（https://arrow.apache.org/docs/index.html）打开。

**RNA .parquet格式文件**
包含7个小鼠脑矢状切面，共检测到168个基因，采样位置为距中线-140μm、600μm、1200μm、1810μm、2420μm、3000μm及3600μm的内外侧位点。
本文件包含所有RNA分子的位置与基因标记信息：其中字段"c_px_microscope_stitched"存储X坐标，"r_px_microscope_stitched"存储Y坐标，单位为像素，单个像素尺寸为0.18微米，乘以0.18即可转换为微米尺度。

**组织轮廓.csv格式文件**
此类CSV文件用于标注7个小鼠图谱切面的样本边界，对应采样位点分别为-140μm、600μm、1200μm、1810μm、2420μm、3000μm及3600μm。部分RNA分子会被检测至组织区域外，若需移除这些异常数据，可在FISHscale中加载数据集并导入该CSV文件中的轮廓信息；若不希望使用FISHscale，也可通过以下代码筛选出组织内的点位：https://github.com/linnarsson-lab/FISHscale/blob/master/FISHscale/utils/inside_polygon.py

**基因颜色.pkl格式文件**
该文件为经Python pickle序列化的字典，存储了本研究小鼠图谱绘图所用的基因对应颜色。