Identification of altered blood microRNAs in a rat model of Parkinson’s disease by Custom "Brain Specific miRNA" Open Array Real-time PCRpanel

12 week male wistar rats were exposed with rotenone (2.5mg/kg b.wt.) and control rats exposed with vehicle (corn oil) intraperetonially for two months. Animals were sacrifised at the end of the experiment and blood was isolated and stored at -80°C in RNALater until further analysis.RNA (including small RNAs) was extracted by Ribopure Blood RNA Isolation Kit by Thermo Fisher as suggested protocol by manufacturer. A custom Taqman OpenArray panel was used to profile the miRNAs expression.将12周龄雄性Wistar大鼠与对照组大鼠均采用腹腔注射方式染毒：实验组给予鱼藤酮（rotenone），剂量为2.5mg/kg体重，对照组给予溶剂（玉米油），持续染毒2个月。实验结束后处死全部实验动物，采集血液并置于RNALater保存液中于-80℃低温储存，以待后续分析。按照赛默飞世尔科技（Thermo Fisher）Ribopure血液RNA提取试剂盒的厂商官方操作流程，提取总RNA（包括小分子RNA）。采用定制化Taqman OpenArray芯片板对微小RNA（miRNAs）的表达谱进行检测分析。