Gene expression analysis of myonectin-regulated genes in atrophic skeletal muscle.

To investigate the role of myonectin in muscle atrophy, myonectin-KO and wild type (C57BL/6J) mice were subjected to sciatic nerve denervation, leading to gastrocnemius muscle atrophy. Overall design: We then performed gene expression profiling analysis using data obained from RNA-seq of 8 gastrocnemius muscle tissues (Myonectin-KO 4, wild type 4).

为探究肌连蛋白(myonectin)在肌肉萎缩中的调控作用，本研究对肌连蛋白基因敲除(myonectin-KO)小鼠与野生型(C57BL/6J)小鼠实施坐骨神经离断术，诱导腓肠肌发生萎缩。实验设计概述：本研究针对8份腓肠肌组织样本（肌连蛋白敲除组、野生型组各4份）的RNA测序(RNA-seq)数据开展基因表达谱分析。