Weight and blood glucose in experimental animals.

The primary objective of this work was to delve into the potential therapeutic advantages and dissect the molecular mechanisms of salidroside in enhancing erectile function in rats afflicted with diabetic microvascular erectile dysfunction (DMED), addressing both the whole-animal and cellular dimensions.We established a DMED model in Sprague‒Dawley (SD) rats and conducted in vivo experiments. The DMED rats were administered varying doses of salidroside, the effects of which on DMED were compared. Erectile function was evaluated by applying electrical stimulation to the cavernous nerves and measuring intracavernous pressure in real time. The penile tissue underwent histological examination and Western blotting. Hydrogen peroxide (H2O2) was employed in the in vitro trial to induce an oxidative stress for the purpose of identifying alterations in cell viability. The CCK-8 assay was used to measure the viability of corpus cavernous smooth muscle cells (CCSMCs) treated with vs. without salidroside. Flow cytometry was utilized to detect alterations in intracellular reactive oxygen species (ROS). Apoptosis was assessed through Western blotting and TdT-mediated dUTP nick-end labelling (TUNEL). Animal and cellular experiments indicate that the Nrf2/HO-1 signalling pathway may be upregulated by salidroside, leading to the improvement of erectile function in diabetic male rats by alleviating oxidative stress and reducing apoptosis in corpus cavernosum tissue.

本研究的核心目标为深入探究红景天苷（salidroside）改善糖尿病微血管性勃起功能障碍（diabetic microvascular erectile dysfunction, DMED）模型大鼠勃起功能的潜在治疗益处，并解析其分子机制，同时从整体动物与细胞两个层面开展相关研究。本研究构建了斯普拉格-道利（Sprague‒Dawley, SD）大鼠糖尿病微血管性勃起功能障碍模型并开展体内实验：向造模成功的DMED大鼠给予不同剂量的红景天苷，对比其对DMED的干预效果；通过对海绵体神经施加电刺激并实时检测海绵体内压，评估大鼠勃起功能；对阴茎组织进行组织学检测与蛋白质印迹（Western blotting）分析。体外实验中，采用过氧化氢（H₂O₂）诱导氧化应激模型以探究细胞活力变化；采用CCK-8法检测经红景天苷处理与未处理的海绵体平滑肌细胞（corpus cavernous smooth muscle cells, CCSMCs）的活力；采用流式细胞术检测细胞内活性氧（reactive oxygen species, ROS）水平变化；通过蛋白质印迹与TdT介导的dUTP缺口末端标记法（TdT-mediated dUTP nick-end labelling, TUNEL）评估细胞凋亡情况。动物与细胞实验结果表明，红景天苷可上调Nrf2/HO-1信号通路，通过减轻海绵体组织的氧化应激与细胞凋亡，进而改善糖尿病雄性大鼠的勃起功能。