DataSheet_1_Cajanin Stilbene Acid Inhibited Vancomycin-Resistant Enterococcus by Inhibiting Phosphotransferase System.docx

Antimicrobial resistance has become a serious threat to human and animal health, and vancomycin-resistant Enterococcus has become an important nosocomial infection pathogen, causing thousands of deaths each year. In this study, after screening a variety of natural products, we found that cajanin stilbene acid (CSA) had significant inhibitory effect on sensitive and vancomycin-resistant Enterococcus (VRE) in vitro. And we also confirmed that CSA had significant anti-VRE infection ability in vivo. Subsequently, we studied the antibacterial mechanism of CSA through proteomics experiments, and the results showed that CSA killed Enterococcus by inhibiting the phosphotransferase system of Enterococcus, thus hinders the normal growth and metabolic functions of bacteria. The results of this study provided evidence for the in-depth study on the mechanism of the antibacterial action of CSA and also provided a candidate for the development of anti-VRE drugs.

抗菌药物耐药性已成为威胁人类与动物健康的严重公共卫生问题，耐万古霉素肠球菌（vancomycin-resistant Enterococcus, VRE）更是已成为重要的医院感染病原菌，每年造成数千人死亡。本研究通过对多种天然产物进行筛选后，发现木豆叶芪类有机酸（cajanin stilbene acid, CSA）在体外对敏感肠球菌以及耐万古霉素肠球菌均具有显著的抑制活性；同时本研究还证实，CSA在体内具备显著的抗VRE感染能力。随后本研究通过蛋白质组学实验探究了CSA的抗菌机制，结果显示CSA通过抑制肠球菌的磷酸转移酶系统来杀灭肠球菌，从而阻碍细菌的正常生长与代谢功能。本研究结果为CSA抗菌作用机制的深入研究提供了科学依据，同时也为抗VRE药物的研发提供了候选化合物。