Additional file 1 of Serum soluble T cell immunoglobulin mucin domain-3 as an early predictive marker for severity of acute pancreatitis; a retrospective analysis

Additional file 1. A total of 94 acute pancreatitis (AP) patients were enrolled in the current analysis, including 42 (45%), 35 (37%), and 17 (18%) patients were diagnosed as mild acute pancreatitis (MAP), moderately severe acute pancreatitis (MSAP), and severe acute pancreatitis (SAP). General information, including gender, age, Etiology (Biliary, Hypertriglyceridemia, Alcoholic , and Other), body mass index (BMI), pre-existing comorbidities (diabetes mellitus, hypertension, and high triglyceride), and substance abuse (alcohol and tobacco) and Length of hospital stay were collected from the medical chart of AP patients (0 means no, 1 means yes). On admission, vital sign (temperature, heart rate, Breathe, oxygen saturation, Mean arterial pressure) were recorded. Blood samples were obtained from AP patients for blood tests within 24 h of admission. Serological tests included serum concentrations of white blood cells (WBCs), red blood cells (RBCs), Hematocrit, platelet, C-reactive protein (CRP), aspartate and alanine aminotransferases (AST and ALT), Total and Direct bilirubin (TB and DB), urea, creatinine, procalcitonin (PCT), and serum activities of amylase. Various severity scoring systems, including acute physiology and chronic health evaluation II (APACHE II) score, bedside index for severity in acute pancreatitis (BISAP) score, and modified computed tomography severity index (MCTSI) score, were made during the first 24 h. Soluble T cell immunoglobulin and mucin domain-3 (sTIM-3), Serum inflammatory cytokine interleukin-6 (IL-6), interleukin-10 (IL-10) levels were determined using the corresponding commercial ELISA kits..

附加文件1。本研究共纳入94例急性胰腺炎（acute pancreatitis, AP）患者，其中42例（45%）、35例（37%）、17例（18%）分别被诊断为轻度急性胰腺炎（mild acute pancreatitis, MAP）、中度重症急性胰腺炎（moderately severe acute pancreatitis, MSAP）及重症急性胰腺炎（severe acute pancreatitis, SAP）。研究人员从AP患者的病历档案中提取了以下一般资料：性别、年龄、病因（胆源性、高甘油三酯血症性、酒精性及其他）、体重指数（body mass index, BMI）、既往合并症（糖尿病（diabetes mellitus）、高血压（hypertension）及高甘油三酯血症）、物质滥用（酒精与烟草）情况以及住院时长（0代表否，1代表是）。患者入院时记录生命体征，包括体温、心率、呼吸频率、血氧饱和度、平均动脉压。于入院24小时内采集AP患者血液样本行实验室检测，血清学检测指标涵盖外周血白细胞（white blood cells, WBCs）、红细胞（red blood cells, RBCs）、血细胞比容、血小板、C反应蛋白（C-reactive protein, CRP）、天冬氨酸转氨酶与丙氨酸转氨酶（AST及ALT）、总胆红素与直接胆红素（TB及DB）、尿素、肌酐、降钙素原（procalcitonin, PCT）以及血清淀粉酶活性。入院后24小时内完成多种重症评分系统评估，包括急性生理学与慢性健康状况评分Ⅱ（acute physiology and chronic health evaluation II, APACHE Ⅱ）、急性胰腺炎重症床旁指数（bedside index for severity in acute pancreatitis, BISAP）以及改良CT严重指数（modified computed tomography severity index, MCTSI）。采用商业化ELISA试剂盒检测可溶性T细胞免疫球蛋白黏蛋白分子-3（soluble T cell immunoglobulin and mucin domain-3, sTIM-3）以及血清炎症因子白细胞介素-6（interleukin-6, IL-6）、白细胞介素-10（interleukin-10, IL-10）的水平。