Fast4DRegistration

<strong>Fast4DReg: Fast registration of 4D microscopy datasets</strong><br> Pylvänäinen J.W et al, 2023 Fast4DReg is a Fiji macro for drift correction in 3D video and is able to correct drift in all x-, y- and/or z-directions. Fast4DReg creates intensity projections along both axes and estimates their drift based on cross-correlation, and then translates the 3D video frame by frame. Additionally, Fast4DReg can be used for aligning multichannel 3D images which is particularly useful for instruments that suffer from a misalignment of channels. GitHub repository: https://github.com/guijacquemet/Fast4DReg Datasets are available in Zenodo: <strong>1 Software archive</strong><br> 1.1 Fast4DReg scripts (5)<br> 1.2 Helper scripts (2)<br> 1.3 Documentation from GitHub <strong>2 Dataset 1: Synthetic drift -datasets</strong><br> 2.1 Synthetic drift files (2)<br> 2.2 Expected results (3) <strong>3 Dataset 2: Noisy images -dataset</strong><br> 3.1 Noisy dataset files (12)<br> 3.2 Average corrected apply output (12)<br> 3.4 Maximum corrected apply output (12) <strong>4 Dataset 3: HUVEC monolayer -dataset</strong><br> 4.1 HUVEC -dataset channel 1 original<br> 4.2 Biological dataset HUVEC channel 1 Fast4DReg<br> 4.3 Biological dataset HUVEC channel 1 C3DD <strong>5 Dataset 4: Mouse lung slice -dataset</strong><br> 5.1 Mouse lung slice original<br> 5.2 Mouse lung slice Fast4DReg <strong>6 Dataset 5: Calibration slide -dataset</strong><br> 6.1 Calibration slide -dataset original<br> 6.2 Calibration slide -dataset Fast4DReg <strong>7 Dataset 6: Filopodia-dataset -dataset</strong><br> 7.1 Filopodia-dataset original<br> 7.2 Filopodia-dataset Fast4DReg <strong>8 Dataset 7: DCIS.com Filopodia -dataset</strong><br> 8.1 DCIS.com Filopodia -dataset original<br> 8.2 DCIS.com Filopodia -dataset Fast4DReg <strong>7 Videos</strong><br> 7.1 Video 1<br> 7.2 Video 2<br> 7.3 Video 3<br> 7.4 Video 4<br> 7.5 Video 5