SH1408197.10FU

UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.

UNITE 提供了一套统一的方法，用于界定、识别、交流以及处理基于 DNA 的物种假说（Species Hypotheses，以下简称 SH）。国际核酸序列数据库中的所有真菌内部转录间隔区（Internal Transcribed Spacer，以下简称 ITS）序列，将通过应用一组动态距离阈值（0.5% 至 3.0%）被聚类至近似物种级别的单元。所有 SH 均会被赋予一个以数字对象标识符（Digital Object Identifier，DOI）形式存在的唯一且稳定的名称，其分类学与生态学注释信息均通过分布式网络第三方注释工作进行验证。各 SH 会结合其包含的全部序列的鉴定结果，尽可能关联至对应的分类单元名称及其分类层级（如门、纲、目等）。科研人员会选取一条经自动或人工指定的序列，作为各 SH 的代表序列。这些代表序列已发布于（https://unite.ut.ee/repository.php），可供科学界用于本地序列相似性搜索、新一代测序分析流程等研究场景。随着公共真菌 ITS 序列数量的持续增长，该系统与配套数据集将自动完成更新。