Proteolytic Regulation of a Galectin-3/Lrp1 Axis Controls Osteoclast-Mediated Bone Resorption

To begin screening global effects on osteoclast activity in an unbiased fashion, we conducted a transcriptome analysis comparing the expression profiles of wild-type, Mmp9/Mmp14 DKO osteoclasts, and Mmp9/Mmp14 DKO osteoclasts treated with GCS-100 Overall design: Comparative gene expression profiling analysis of RNA-seq data for wild-type, Mmp9/Mmp14 DKO osteoclasts, and Mmp9/Mmp14 DKO osteoclasts treated with GCS-100.

为以无偏倚的方式全面筛选破骨细胞活性的全局调控效应，本研究开展转录组分析，对比野生型、基质金属蛋白酶9/基质金属蛋白酶14双基因敲除（Mmp9/Mmp14 DKO）破骨细胞，以及经GCS-100处理的Mmp9/Mmp14 DKO破骨细胞的基因表达谱。实验设计：对野生型、Mmp9/Mmp14 DKO破骨细胞，以及经GCS-100处理的Mmp9/Mmp14 DKO破骨细胞的RNA测序（RNA-seq）数据开展比较基因表达谱分析。