Transcriptome-based exon capture of Harmonia axyridis
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https://www.ncbi.nlm.nih.gov/sra/ERP114162
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Two target enrichment experiments were performed with the same individuals, the first capture with 23 indexed multiplexed individuals and the second with a pool of 36 non-indexed individuals. Capture probes were designed for ~5 Mb of randomly chosen coding exonic regions of H. axyridis from a de novo transcriptome (Vogel et al. 2017). Target enrichment using SeqCap EZ Developer Library version 5.0 (Roche Nimbelgen Technologies). DNA extractions were performed using Qiagen Dneasy Blood & Tissue kit. For more details, see https://www.biorxiv.org/content/10.1101/583534v1 .
本研究针对同一批实验个体开展了两次靶标富集实验：首轮捕获使用23个已索引的多重化个体，次轮则采用由36个未索引个体组成的混合样本。捕获探针针对异色瓢虫（Harmonia axyridis）约5 Mb的随机筛选编码外显子区域设计，该区域数据源自一项从头转录组研究（Vogel等，2017）。靶标富集流程采用罗氏NimbleGen技术公司的SeqCap EZ Developer Library 5.0版本试剂盒执行。DNA提取使用Qiagen Dneasy血液与组织试剂盒完成。更多详细信息可参阅：https://www.biorxiv.org/content/10.1101/583534v1
创建时间：
2019-03-23
